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Fink K, Clark B
Am Fam Physician. 2003 May 1; 67(9):1975-6.

[ PubMed ] [ Ref list ]

Despite the broader distribution of Sec61 and OST complex proteins in adrenal microsomes, two features distinguished isolated adrenal rough from smooth microsomes: bound ribosomes and αTRAP. These features are shared with rough microsomes prepared from pancreas and liver and are consistent with active cotranslational translocation ( 30 ).

However, it seems that there are at least two forms of SER. One, found in steroid-secreting cells, possesses abundant machinery for the translocation and processing of proteins targeted for the ER. The other, characteristic of protein-secreting cells, does not. The high levels of these proteins in adrenal smooth microsomes cannot be attributed to contamination by rough microsomes. The small amount of RER present in adrenocortical cells and the clear localization of ribosomal protein to the rough microsomal fraction preclude this as does their distribution throughout the ER visualized by immunocytochemistry. Furthermore, the broader distribution of these proteins in adrenal ER is not related to the amount of SER per se . Smooth microsomes from livers of animals treated with xenobiotics that expand the SER in hepatocytes did not contain high levels of these proteins.

Although not as dramatic as the data presented here, there have been a few previous reports of smooth endomembrane systems possessing RER-specific proteins in other cell types. Small amounts of translocon-associated proteins have been seen in SER found in mammalian dendrites (Sec61α) ( 82 ) and Caenorhabditis elegans neurites [ribosome-associated membrane protein 4 (RAMP4), TRAPβ, translocating-chain associating membrane (TRAM)] ( 83 ). However, as shown in C. elegans , the bulk of the ribosomes and translocon-associated proteins colocalize in the neuron cell bodies that contain abundant RER ( 83 ). In studies using less specific reagents, an antibody made against RER membrane proteins reacted with the sarcoplasmic reticulum in skeletal muscle and smooth-surfaced cisternal stacks in Purkinje neurons ( 84 , 85 ). These compartments both contain high levels of specific multispanning transmembrane (TM) proteins (Ca 2 +ATPase and inositol 1,4,5-trisphosphate receptor, respectively) (for discussion of these and other ER domains, see Ref. 86 ).

We hypothesize that the components of the translocation apparatus found in adrenal smooth microsomes participate in synthesis of membrane proteins targeted for particular domains in the ER and/or quality control of these proteins. Morphologically distinct domains clearly exist in adrenocortical cells ( 6 , 7 ) and smaller domains of enzyme complexes have been postulated ( 87 ). Two lines of evidence suggest some correlation with cholesterol production. First, translocation components were more prominent in adrenal smooth microsomes of species in which the adrenals synthesize a large proportion of their own cholesterol ( 88 91 ). They were less prominent in SER of the rat adrenal that depends on plasma lipoproteins, rather than de novo synthesis, for cholesterol used in steroid synthesis ( 89 , 90 ). Second, Sec61 and OST were not as prominent in the equally abundant SER induced in hepatocytes by xenobiotics. These SER membranes showed relative increases in CYPs involved in metabolism of foreign compounds but did not show a relative increase in the concentration of HMGR.

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Gilbert and Sullivan scored their first international hit with H.M.S. Pinafore (1878), satirising the rise of unqualified people to positions of authority and poking good-natured fun at the Royal Navy and the English obsession with social status (building on a theme introduced in The Sorcerer , love between members of different social classes). As with many of the Gilbert and Sullivan operas, a surprise twist changes everything dramatically near the end of the story.

Gilbert oversaw the designs of sets and costumes, and he directed the performers on stage. [30] He sought realism in acting, shunned self-conscious interaction with the audience, and insisted on a standard of characterisation where the characters were never aware of their own absurdity. Womens Spring Cross Over Platform Heels Dorothy Perkins JNdvzbBw
Gilbert insisted that his actors know their words perfectly and obey his stage directions, which was something new to many actors of the day. [31] Sullivan personally oversaw the musical preparation. The result was a new crispness and polish in the English musical theatre. Womens Ruthin ClosedToe Pumps Gabor ViMvdE3
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As Jessie Bond wrote later:

Our stage discipline was strict and unbending. Gilbert's word was law; he thoroughly worked out in his own mind every bit of action, by-play and grouping, and allowed no deviation from his plan. He...made drawings and took measurements with the minutest care.... He had unlimited fertility of invention in comic business and would allow no gag, no clowning, no departure from his own definite conception. Sullivan's musical conception was equally clear-cut and decided. Every part must be made subservient to the whole, and his sarcasms overwhelmed the transgressor with scorn. "And now, might I trouble you to try over my music," he would say to a singer too anxious to display his or her top notes. But there was nothing to hurt or offend us in this unswerving discipline, we took their good-humoured raillery as our due when we failed in our rendering or overstepped the bounds; and the patience and enthusiasm of that artistic pair so infected all of us that we worked willingly for hours and hours at rehearsals, trying with all our might to realize the conceptions of those two brilliant minds. DENIM Denim trousers Be Able Infinity And Beyond 91UGsFx

H.M.S. Pinafore ran in London for 571 performances, [35] the second longest run of any musical theatre piece in history up to that time (after the operetta Les cloches de Corneville ). [36] Hundreds of unauthorised, or "pirated", productions of Pinafore appeared in America. Peony bow slingback Black Salvatore Ferragamo 8Qm2ifxk
During the run of Pinafore , Richard D'Oyly Carte split up with his former investors. The disgruntled former partners, who had invested in the production with no return, staged a public fracas, sending a group of thugs to seize the scenery during a performance. Stagehands successfully managed to ward off their backstage attackers. [38] This event cleared the way for Carte, Gilbert and Sullivan to form the D'Oyly Carte Opera Company, which then produced all of their succeeding operas.

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President Donald Trump and first lady Melania Trump leave Winfield House, residence of the U.S. Ambassador, before boarding Marine One helicopter for the flight to nearby Blenheim Palace, Thursday, July 12, 2018, in London. (AP Photo/Pablo Martinez Monsivais)

Melania Trump has been turning heads in her latest fashion choice at the black-tie dinner hosted by Prime Minister Theresa May at Blenheim Palace Thursday evening, marking Trumps’ first official visit to Britain.

MELANIA TRUMP’S STATE OF THE UNION OUTFIT: PERSONAL STATEMENT, OR JUST A NICE SUIT?

FLOTUS stood out with a pale yellow chiffon gown from French label J. Mendel. (Associated Press)

While President Trump sported a more traditional black-and-white tuxedo, FLOTUS stood out with a pale yellow chiffon gown from French label J. Mendel. The off-shoulder dress featured a wrapped bodice, floor-length pleats and a billowing cape that gave her a dramatic, yet delicate look.

Melania paired the ensemble with minimal jewelry and sky-high stiletto heels in a matching pastel color.

The first lady has been praised for her gown across social media. (Associated Press)

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After Melania debuted the gown, several took to Twitter to gush about the outfit, even comparing the first last to a Disney princess.

Melania's dress is just beautiful she looks like a princess

Radiant Melania is reminiscent of a Disney princess in a yellow off the shoulder gown: qua

can I borrow that yellow dress please. Super please!!!! Stunning!!!!

That dress on tho, she is so hot

I love Melania’s dress!!! 😍😍😍

While the dress seemed to be a big hit with most, some were not as complimentary of the buttery yellow frock.

Sorry but as pretty as it is Melania's yellow dress is WAAY too formal for a dinner dress. Looks like she's going to a royal wedding or a presidential prom.

I knew Melania's awful dress reminded me of someone. How apt!

Melania's dress looks ridiculous and old fashioned.

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